Conditioned Medium from Human Adipose-Derived Mesenchymal Stromal Cells Can Modulate Cell Migration and Morphology of Keratinocytes In Vitro

February 2026 in “ Human Cell ”

Cristiano Rodrigues, Thaís Casagrande Paim, Carla Zanatelli, Elisa Vasconcellos Soares Prignon, Jéssica Gonçalves Azevedo, Liliana Ivet Sous Naasani, Márcia Rosângela Wink

conditioned medium human adipose-derived mesenchymal stromal cells keratinocytes TGF-β1 actin stress fibers filopodia protrusions cell migration cell morphology skin repair dermal regeneration therapies MSC-CM mesenchymal stromal cells transforming growth factor beta 1

TLDR MSC-CM can boost skin cell growth and movement, aiding skin repair.

This study investigated the effects of conditioned medium from human adipose-derived mesenchymal stromal cells (MSC-CM) on keratinocytes, specifically focusing on cell migration and morphology. The MSC-CM was found to enhance keratinocyte proliferation and migration, as well as induce changes in cell morphology, such as increased actin stress fibers and filopodia protrusions. The modulation of keratinocyte dynamics was partly attributed to TGF-β1 signaling, as blocking this pathway significantly reduced cell migration. These findings highlight the potential of MSCs in promoting skin repair and their role in dermal regeneration therapies.

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