Development of High-Performance Two-Dimensional Gel Electrophoresis for Human Hair Shaft Proteome

March 2019 in “ PLoS ONE ”

Sing Ying Wong, Onn Haji Hashim, Nobuhiro Hayashi

two-dimensional gel electrophoresis 2DE hair shaft proteome protein isolation biomarkers hair health hair diseases

TLDR A new method improves protein analysis in hair, aiding health and disease research.

The study developed a high-performance protocol for analyzing the human hair shaft proteome using two-dimensional gel electrophoresis (2DE). By employing an alkaline-based method for protein isolation, the researchers introduced both conventional and modified proteomic protocols, detecting over 400 protein spots. The modified protocol improved reproducibility and provided a comprehensive overview of protein distribution. This advancement aimed to facilitate hair analysis and potentially lead to the discovery of biomarkers related to hair health and diseases.

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Research cited in this study

8 / 8 results

research Characterization of the Promoter Regions of Two Sheep Keratin-Associated Protein Genes for Hair Cortex-Specific Expression

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Development of High-Performance Two-Dimensional Gel Electrophoresis for Human Hair Shaft Proteome

Research cited in this study

research Characterization of the Promoter Regions of Two Sheep Keratin-Associated Protein Genes for Hair Cortex-Specific Expression

research Human Hair Shaft Proteomic Profiling: Individual Differences, Site Specificity, And Cuticle Analysis

research A Novel Purification Procedure for Keratin-Associated Proteins and Keratin from Human Hair

research Proteome Analysis of Human Hair Shaft

research Apoptosis and Keratin Intermediate Filaments

research A Rapid Extraction Procedure of Human Hair Proteins and Identification of Phosphorylated Species

research The Role of Keratin Proteins and Their Genes in the Growth, Structure, and Properties of Hair

research Sequence, Expression, and Evolutionary Conservation of a Gene Encoding a Glycine/Tyrosine-Rich Keratin-Associated Protein of Hair

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research Potential Use of Human Hair Shaft Keratin Peptide Signatures to Distinguish Gender and Ethnicity