Development of High-Performance Two-Dimensional Gel Electrophoresis for Human Hair Shaft Proteome
March 2019
in “
PLoS ONE
”
Research cited in this study
8 / 8 resultsresearch Characterization of the Promoter Regions of Two Sheep Keratin-Associated Protein Genes for Hair Cortex-Specific Expression
The KRTAP11-1 gene promoter is crucial for specific expression in sheep wool cortex.
research Human Hair Shaft Proteomic Profiling: Individual Differences, Site Specificity, And Cuticle Analysis
Human hair proteins vary by individual, body site, and ethnicity, useful for forensics.
research A novel purification procedure for keratin-associated proteins and keratin from human hair
The study developed a novel purification procedure for keratin-associated proteins (KAPs) and keratin from human hair using alcohols in the Shindai solution, which enhanced KAPs extraction while suppressing keratin extraction. The procedure, particularly with ethanol, allowed for the selective purification of KAPs and keratin, identifying six KAPs polypeptides and two keratin polypeptides. The amino acid compositions matched literature values, with recovery rates of approximately 10% for KAPs and 50% for keratin. Scanning electron microscopy revealed that KAPs may support keratin fibers by surrounding fibrous structures in the hair cortex, which disappeared upon keratin extraction.
research Proteome Analysis of Human Hair Shaft
The study conducted a proteome analysis of the human hair shaft using two-dimensional LC-MS/MS, identifying 343 proteins. Among these, 70 proteins, including keratin intermediate filament proteins, were found in high abundance and were largely extractable with denaturants. Over 300 proteins were part of an insoluble complex formed by transglutaminase cross-linking. The proteins identified were distributed across various cellular locations, including the cytoplasm, nucleus, mitochondria, ribosome, and plasma membrane. The study provided insights into the ultrastructural features of mature hair, noting posttranslational modifications such as methylation, dimethylation, and evidence of ubiquitination in hair proteins.
research Apoptosis and keratin intermediate filaments
research A Rapid Extraction Procedure of Human Hair Proteins and Identification of Phosphorylated Species.
A new method quickly extracts and identifies proteins from hair and other keratin sources.
research The role of keratin proteins and their genes in the growth, structure and properties of hair
research Sequence, expression, and evolutionary conservation of a gene encoding a glycine/tyrosine-rich keratin-associated protein of hair.
Related Research
1 / 1 resultsresearch Potential Use of Human Hair Shaft Keratin Peptide Signatures to Distinguish Gender and Ethnicity
The study explored the potential of using keratin peptide signatures from human hair shafts to distinguish gender and ethnicity, as traditional DNA analysis is often not possible with hair samples from old crime scenes. Researchers extracted and analyzed hair shaft proteins from 40 healthy individuals using gel electrophoresis-based proteomics. They found significant differences in the abundance of specific keratin peptides (K81, K83, K86) between genders and among ethnic groups in Malaysia. These findings suggested that keratin peptide profiles could potentially serve as forensic markers for gender and ethnicity, although further research with a larger sample size was needed to confirm these results.