TLDR A new method effectively separates keratin-associated proteins and keratin from human hair.
The study developed a novel purification procedure for keratin-associated proteins (KAPs) and keratin from human hair using alcohols in the Shindai solution, which enhanced KAPs extraction while suppressing keratin extraction. The procedure, particularly with ethanol, allowed for the selective purification of KAPs and keratin, identifying six KAPs polypeptides and two keratin polypeptides. The amino acid compositions matched literature values, with recovery rates of approximately 10% for KAPs and 50% for keratin. Scanning electron microscopy revealed that KAPs may support keratin fibers by surrounding fibrous structures in the hair cortex, which disappeared upon keratin extraction.
3 citations
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May 2012 in “Journal of Biological Macromolecules” Keratin film can effectively mimic human hair for testing hair damage.
52 citations
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April 2012 in “Journal of Investigative Dermatology” KRTAP2 genes are crucial for hair structure and may impact hair disorders and treatments.
10 citations
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January 2012 in “Journal of Biological Macromolecules” Keratin film can effectively replace human hair for testing hair damage.
517 citations
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February 2010 in “Materials” Keratin from hair and wool is used in medical materials for healing and drug delivery.
226 citations
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January 2006 in “International review of cytology” 226 citations
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January 2002 in “Biological and Pharmaceutical Bulletin” A new method quickly extracts and identifies proteins from hair and other keratin sources.
272 citations
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September 2001 in “Journal of Biological Chemistry” Human hair keratins were cataloged, showing their roles in hair differentiation stages.
235 citations
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July 1999 in “Journal of biological chemistry/The Journal of biological chemistry” Human hair is made up of different keratins, some strong and some weak, with specific types appearing at various stages of hair growth.
85 citations
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January 1990 5 citations
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January 1990 in “Journal of Society of Cosmetic Chemists of Japan”
8 citations
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May 2020 in “International journal of biological macromolecules” Certain treatments can increase protein binding to natural hair but are less effective on permed hair.
Keratin hydrogels from human hair show promise for tissue engineering and regenerative medicine.
Human hair keratins can self-assemble and support cell growth, useful for biomedical applications.
Human hair keratins can be turned into useful 3D biomedical scaffolds through a freeze-thaw process.
41 citations
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July 2020 in “Colloids and surfaces. B, Biointerfaces” Different hair protein amounts change the strength of keratin/chitosan gels, useful for making predictable tissue engineering materials.
