1 citations
,
July 2023 in “Psychoneuroendocrinology” This study found that hair cortisol concentration is consistently associated with salivary cortisol output throughout the day, making it a potential reliable biomarker of long-term cortisol secretion.
19 citations
,
June 2020 in “General and comparative endocrinology” This study found that hair cortisol concentration is a promising stress indicator in cattle but not in pigs, where external factors and lower cortisol response may interfere with its validity.
January 2017 in “Qucosa (Saxon State and University Library Dresden)” This study found that plasma protein binding significantly contributes to the shifts in glucocorticosteroid concentration ratios among blood, saliva, and hair, challenging prior assumptions about enzyme inactivation as the main factor.
9 citations
,
April 2016 in “Clinical Endocrinology” This study suggests that measuring scalp hair 17-hydroxyprogesterone and androstenedione concentrations can be a promising tool for monitoring treatment in children with congenital adrenal hyperplasia.
July 2025 in “International Journal of Dermatology Venereology and Leprosy Sciences” In this review, researchers examined the concentration of cortisol in scalp hair of individuals with alopecia areata to explore its relationship with disease variables, as cortisol in hair can indicate chronic stress, which may impact alopecia areata pathogenesis.
April 2026 in “Frontiers in Veterinary Science” This study observed that in privately owned, intact cats, male cats exhibited significantly higher long-term hair cortisol concentrations compared to females, while environmental and behavioral factors did not significantly impact cortisol levels, suggesting individual traits may influence stress markers more than external factors.
June 2020 in “Authorea (Authorea)” This study observed that pregnant women experienced an increase in hair cortisol concentrations, with primiparae having significantly higher levels in the last trimester compared to multiparae.
50 citations
,
November 2020 in “Physiology & behavior” This review found that patients with depression generally have higher hair cortisol concentrations compared to healthy controls, while those with PTSD tend to have lower concentrations.
February 2026 in “Psychoneuroendocrinology” Hair cortisol concentration partly reflects HPA axis regulation but doesn't capture all its complexities.
January 2026 in “Psychoneuroendocrinology” Lye relaxers don't significantly change hair cortisol levels.
May 2023 in “Animal Reproduction Update” This article reviews the use of hair cortisol concentration as a biomarker to assess long-term stress in both animals and humans, but it reports no new experimental results.
11 citations
,
April 2020 in “Animals” This study reported that relocation in police horses was associated with elevated hair cortisol concentrations, possibly suggesting altered welfare due to changed surroundings, and that seasonal variations affected both cortisol levels and hair growth.
118 citations
,
October 2014 in “Journal of Dairy Science” This study found that in lactating Holstein cows, hair from the tail switch, which grows faster and is more sensitive to cortisol changes, is ideal for measuring hair cortisol levels.
33 citations
,
January 2017 in “Psychoneuroendocrinology” This study of Danish women found no evidence linking hair cortisol concentration to hair dyeing status or the frequency of hair washing.
24 citations
,
October 2018 in “Addiction Biology” This study found that alcohol dependence is associated with altered HPA axis functioning, with AD patients showing higher hair cortisol concentrations and reduced ACTH and cortisol levels, irrespective of childhood maltreatment history.
22 citations
,
August 2016 in “European Eating Disorders Review” This study found that female patients with anorexia nervosa had lower hair cortisol concentration compared to healthy and psychiatric controls, which may be influenced by body mass index rather than anorexia-related endocrine changes.
21 citations
,
January 2017 in “MethodsX” This study found that methanol is more effective than isopropanol at removing external cortisol from grizzly bear hair and suggests that the choice of solvent can influence hair cortisol concentration results.
122 citations
,
October 2010 in “Clinica Chimica Acta” In this study, washing rhesus monkey hair with water or shampoo significantly reduced cortisol levels, suggesting potential underestimation of chronic stress hormone deposition in hair due to washing.
90 citations
,
July 2014 in “Conservation Physiology” This study found that hair cortisol concentration in free-ranging brown bears is influenced by handling methods, body condition, and potentially both short- and long-term stressors.
October 2025 in “Journal of Veterinary Diagnostic Investigation” This study found that in domestic cats, black hair contained significantly higher cortisol concentrations than white hair, whereas ginger hair showed no significant difference compared to white hair. Additionally, a positive correlation was reported between hair cortisol levels and behavioral issues mentioned by cat owners.
This study found that women undergoing mid-trimester termination of pregnancy exhibited increased cumulative cortisol and testosterone levels in hair samples, higher anxiety, but better stress coping strategies two months post-abortion, suggesting hair testosterone may be linked to emotional vulnerability such as neuroticism during this period.
22 citations
,
January 2010 in “Clinical Medicine Insights Endocrinology and Diabetes” In this study, hair from forearms showed higher cortisol levels than hair from lower legs, suggesting a localized cortisol response in hair.
69 citations
,
May 2016 in “General and Comparative Endocrinology” This study found that hair cortisol concentrations in sheep were significantly increased by extensive brushing or dexamethasone treatment, suggesting that external factors may affect its reliability as a chronic stress measure.
3 citations
,
November 2021 in “Stress” This study found that school-related stress increases hair cortisol in girls and alters hair testosterone and cortisol levels in boys, indicating gender-specific hormonal responses to environmental changes.
This study reports that hair samples can be used to track chronic hormone levels, revealing stress, reproductive, and sodium regulation patterns in wildebeest related to environmental factors.
157 citations
,
June 2015 in “Psychoneuroendocrinology” This study observed that hair cortisol and cortisone concentrations were higher in older individuals and those with diabetes mellitus, highlighting their potential significance for assessing somatic health.
August 2021 in “Asian Journal of Pharmaceutical and Clinical Research” In this randomized trial, a solid shampoo formulated with plant extracts was more effective than placebo in improving androgenetic alopecia and reducing hair cortisol and DHT concentrations in men.
10 citations
,
September 2019 in “Frontiers in Veterinary Science” This study found that older steers had higher hair cortisol concentrations compared to younger steers, suggesting age affects chronic stress biomarkers in beef cattle.
May 2023 in “General and comparative endocrinology” This study found that hair cortisol concentrations in rhesus macaques were not significantly correlated with hair growth rate, suggesting that cortisol-mediated inhibition of hair growth does not explain higher cortisol levels within the non-stress range.
January 2018 in “Digital Scholarship - UNLV (University of Nevada Reno)” This study found significantly higher hair cortisol concentrations in black children compared to white children aged 1-4 years, suggesting racial differences in cumulative stress as measured by hair cortisol.