Comparison of Methanol and Isopropanol as Wash Solvents for Determination of Hair Cortisol Concentration in Grizzly Bears and Polar Bears

    January 2017 in “ MethodsX ”
    Thomas Kroshko, Luciene Kapronczai, Marc Cattet … David M. Janz
    Studysummary This study found that methanol is more effective than isopropanol at removing external cortisol from grizzly bear hair and suggests that the choice of solvent can influence hair cortisol concentration results.
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    Research cited in this study 7

    1. Sources of Variation in Hair Cortisol in Wild and Captive Non-Human Primates Zoology · 2016
    2. Analysis of Hair Cortisol Levels in Captive Chimpanzees: Effect of Various Methods on Cortisol Stability and Variability MethodsX · 2016
    3. Quantifying Long-Term Stress in Brown Bears with Hair Cortisol Concentration: A Biomarker That May Be Confounded by Rapid Changes in Response to Capture and Handling Conservation Physiology · 2014
    4. Correlation of Cortisol in 1-Cm Hair Segment with Salivary Cortisol in Humans: Hair Cortisol as an Endogenous Biomarker Clinical Chemistry and Laboratory Medicine (CCLM) · 2011
    5. Prenatal Hair Development: Implications for Drug Exposure Determination Forensic science international · 2010
    6. Cortisol Determination in Hair and Feces from Domestic Cats and Dogs General and Comparative Endocrinology · 2007
    7. On Pathways for Small Molecules Into and Out of Human Hair Fibers Journal of Forensic Sciences · 1996