The Formulation and In-Vitro Evaluation of WS Biotin, a Novel Encapsulated Form of D-Biotin with Improved Water Solubility for Hair and Skin Treatment Applications

October 2023 in “ International Journal of Cosmetic Science ”

Serena Duchi, Paloma Rebollo Torregrosa, Akram Hajuj, Darius Molho, Rawya Shkoor, Nadeen Abo Saada, David González Fernández, D. Goldstein, Alejandro Pérez‐Fernández

WS Biotin D-Biotin follicular keratinocyte proliferation keratins KRT40 keratins KRT85 VEGF FGF7 IGF1 hair growth-related genes hair maintenance hair shaft strength skin-lightening melanin levels biotin hair growth skin treatment

TLDR WS Biotin, a new form of D-Biotin, improves water solubility and shows potential for hair and skin care without being toxic at low levels.

The study developed and evaluated WS Biotin, a new encapsulated form of D-Biotin with 40 times improved water solubility, for use in hair and skin treatments. The microcapsules contained 46.1% D-Biotin and showed no significant cytotoxicity at concentrations below 0.1%. WS Biotin was found to promote follicular keratinocyte proliferation, enhance the expression of hair-associated keratins KRT40 and KRT85, and upregulate the expression of hair growth-related genes VEGF, FGF7, and IGF1, suggesting its potential in promoting hair maintenance and improving hair shaft strength. Additionally, WS Biotin demonstrated a potential skin-lightening effect by triggering a dose-dependent decrease in melanin levels. The study indicates the need for enhanced delivery strategies for biotin due to the high doses used.

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