Volume Imaging to Interrogate Cancer Cell-Tumor Microenvironment Interactions in Space and Time

May 2023 in “ Frontiers in Immunology ”

Jorge Almagro, Hendrik A. Messal

Volume imaging, through technologies like tissue clearing and intravital microscopy (IVM), has significantly advanced the understanding of cancer cell interactions within the tumor microenvironment (TME). These methods allow for high-resolution, 3D visualization of tumors and their dynamic interactions over time, providing 4D data. This has enhanced knowledge of tumor architecture, stromal and immune infiltration, vascularization, and innervation, and has opened new avenues for immunotherapy and optimized chemotherapy delivery. The review highlights the impact of these imaging technologies on both fundamental cancer research and clinical oncology.

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