A Doxycycline- and Light-Inducible Cre Recombinase Mouse Model for Optogenetic Genome Editing

    October 2022 in “ Nature Communications ”
    Miguel Vizoso, Colin E.J. Pritchard, Lorenzo Bombardelli, Bram van den Broek, Paul Krimpenfort, Roderick L. Beijersbergen, Kees Jalink, Jacco van Rheenen
    TLDR The DiLiCre mouse model is an effective tool for precise genome editing using light.
    The study developed an optimized photoactivatable Cre recombinase system called DiLiCre, which was both doxycycline- and light-inducible. This system was characterized in cancer cell and organoid systems, and a DiLiCre mouse line was generated to demonstrate its biological applicability. The research showed that DiLiCre could be used for light-induced mutagenesis in vivo and positional cell-tracing through intravital microscopy. The experiments revealed that newly formed HrasV12 mutant cells exhibited unnatural movement towards the interfollicular dermis. Overall, the DiLiCre mouse model was established as an efficient and powerful tool for genome editing in biological and biomedical research.
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