Study of Constructing Tissue Engineered Urethra Membrane Using Hair Follicle Stem Cells and Nano Silk Fiber In Vitro

The study explored the feasibility of constructing a tissue-engineered urethra membrane using rabbit hair follicle stem cells and nano silk fibers. Hair follicle stem cells were isolated, cultured, and then seeded on electro-spun nano silk fibers. The results showed that the stem cells had a high amplification factor and colony forming rate, with significant expression of K19, p63, and CD29 markers. When seeded on nano silk fibers, the stem cells formed a stratified epithelium-like structure, confirmed by positive AE1/AE3 staining. The study concluded that combining rabbit hair follicle stem cells with nano silk fibers successfully constructed a tissue-engineered urethra, suggesting potential for future in vivo repair studies.