A New Method for Culturing Human Hair Follicle Cells on Floating Mixed Collagen Membranes

    January 1990 in “ The Journal of Dermatology ”
    Ryuichiro Kuwana, Seiji Arase, Yasushi Sadamoto, Katsuyuki Takeda
    TLDR Human hair follicle cells can be grown successfully on floating collagen membranes without extra support.
    A new method was developed for culturing human hair follicle cells on floating mixed collagen membranes without a biological feeder layer. Hair follicle cells from plucked hairs were successfully grown and reached confluence, allowing for subculturing up to two times. The cells maintained differentiation for a month, showing stratification and epithelial pavement formation similar to cultured epidermal keratinocytes.
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