A New Method for Culturing Human Hair Follicle Cells on Floating Mixed Collagen Membranes

January 1990 in “ The Journal of Dermatology ”

Ryuichiro Kuwana, Seiji Arase, Yasushi Sadamoto, Katsuyuki Takeda

human hair follicle cells floating mixed collagen membranes collagen type I collagen type IV epithelial pavement formation cultured epidermal keratinocytes

TLDR Human hair follicle cells can be grown successfully on floating collagen membranes without extra support.

The study introduced a novel method for culturing human hair follicle cells without the need for a biological feeder layer. Researchers successfully grew dispersed hair follicle cells from plucked hairs on floating mixed collagen membranes composed of collagen types I and IV. These cells reached a confluent state and could be subcultured up to two times. The differentiation of the cells was maintained for a month in culture, and the cells exhibited stratification and continuous epithelial pavement formation, similar to cultured epidermal keratinocytes.

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