Dysregulation of Leukemia Inhibitory Factor (LIF) Signaling Pathway by Supraphysiological Dose of Testosterone in Female Sprague Dawley Rats During Development of Endometrial Receptivity

January 2025 in “ Biomedicines ”

Allia Najmie Muhammad Yusuf, Mohd Fariz Amri, Azizah Ugusman, Adila A. Hamid, Izzat Zulhilmi Abd Rahman, Mohd Helmy Mokhtar

testosterone Leukaemia Inhibitory Factor LIF LIFR JAK1 STAT3 MUC1 finasteride anastrozole Propecia Arimidex

Studysummary In this study, female rats given high doses of testosterone showed decreased endometrial thickness and lower mRNA levels of components in the LIF signalling pathway, indicating that excessive testosterone may negatively impact endometrial receptivity and potentially affect fertility.

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The study on 30 female Sprague Dawley rats examined the effects of a supraphysiological dose of testosterone on the LIF signaling pathway and endometrial receptivity. Testosterone treatment significantly reduced endometrial and myometrial thickness, decreased the number of glands, and downregulated mRNA levels of LIF, LIFR, JAK1, and STAT3, while upregulating MUC1. These changes suggest that high testosterone levels impair endometrial receptivity by disrupting the LIF signaling pathway, potentially affecting fertility. The combination of testosterone with inhibitors like finasteride and anastrozole did not fully counteract these effects, highlighting the need for further research on androgenic impacts on fertility.

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