Sub-Chronic Testosterone Treatment Increases the Levels of Epithelial Sodium Channel (ENaC)-α, β, and γ in the Kidney of Orchidectomized Adult Male Sprague–Dawley Rats

The study investigated the effects of testosterone on the expression of α, β, and γ-epithelial sodium channel (ENaC) proteins and mRNAs in the kidneys of orchidectomized adult male Sprague–Dawley rats. Testosterone treatment increased the levels of these ENaC proteins and mRNAs, particularly in the distal tubules and collecting ducts, which could enhance Na+ reabsorption and potentially raise blood pressure. Co-treatment with flutamide or finasteride reduced these levels, suggesting that testosterone's influence on ENaC expression might contribute to its effect on blood pressure.