Testosterone Enhances Expression and Functional Activity of Epithelial Sodium Channel, Cystic Fibrosis Transmembrane Regulator, and Sodium Hydrogen Exchanger in Vas Deferens of Sex-Steroid Deficient Male Rats

July 2018 in “ Steroids ”

Norlisah Ramli, Nelli Giribabu, Naguib Salleh

testosterone epithelial sodium channel ENaC cystic fibrosis transmembrane regulator CFTR sodium hydrogen exchanger NHE vas deferens flutamide finasteride amiloride Cftr inh-172 ion transporters γ-ENaC NHE-1 NHE-4 NHE-2 NHE-3

TLDR Testosterone changes how certain channels work in male rat reproductive tracts, affecting fertility-related fluid balance.

The study investigated the effects of testosterone on the expression and functional activity of epithelial sodium channel (ENaC), cystic fibrosis transmembrane regulator (CFTR), and sodium hydrogen exchanger (NHE) in the vas deferens of sex-steroid deficient male rats. Testosterone treatment increased the secretion rate and concentrations of Cl− and Na+ while decreasing pH and HCO3− concentrations in the vas deferens fluid. These effects were counteracted by amiloride, Cftr inh-172, flutamide, and finasteride. The study concluded that testosterone enhances the expression of γ-ENaC, CFTR, and NHE isoforms in the vas deferens epithelium, indicating their crucial role in maintaining the fluid microenvironment necessary for male fertility.

View this study on sciencedirect.com →

Discuss this study in the Community →

Research cited in this study

2 / 2 results

research Testosterone Up-Regulates Vacuolar ATPase Expression and Functional Activities in Vas Deferens of Orchidectomized Rats

2 citations, December 2017 in “Theriogenology”

Testosterone increases acidification in the vas deferens by up-regulating V-ATPase.

research Effects of Testosterone on Mean Arterial Pressure and Aquaporin Expressions in the Kidney of Orchidectomized Adult Male Sprague-Dawley Rats

9 citations, January 2017 in “Archives of biochemistry and biophysics”

Testosterone raises blood pressure and changes kidney protein levels.

Related Community Posts Join

6 / 1000+ results

community Finasteride only reduces scalp DHT by ~40%. Why does it work?

in Finasteride/Dutasteride 37 upvotes 1 year ago

How Finasteride and Dutasteride help reduce hair loss by reducing the amount of DHT in scalp areas, as well as discussing factors such as sensitivity to DHT, 5α-reductase expression in different parts of the hair follicle, and scalp tension.

community Evidenced Based Meta-Analyses and Systemic Reviews of Androgenic Alopecia and Treatments

in Research/Science 14 upvotes 4 years ago

Evidence-based treatments for androgenic alopecia, such as minoxidil, finasteride, low-level laser light therapy, dutasteride, platelet-rich plasma, and topical ketoconazole. It discusses the efficacy, safety, and mechanism of action of these treatments, as well as future developments in understanding this polygenic condition.

community Why is Minoxidil not enough for hair loss? (No fin)

in Minoxidil 57 upvotes 6 days ago

Minoxidil alone is often insufficient for treating hair loss because it doesn't address the DHT-related cause. Combining it with finasteride, a DHT blocker, is generally more effective.

community Is it possible for androgenic alopecia to begin at early adolescent years?

in Chat 70 upvotes 1 year ago

Hair loss can begin in early adolescence and cause mental anguish. Treatments mentioned include eating cruciferous vegetables, engaging in physical activity, and maintaining scalp hygiene.

community This is the best time in human history to be fighting hair loss

in Treatment 193 upvotes 2 years ago

This conversation discusses the advancements in treatments for hair loss, including finasteride, dutasteride, minoxidil and new promising treatments such as pyrilutamide and verteporfin. Other treatments discussed were taking minoxidil orally, tretinoin to turn non-responders into responders and microneedling to further boost growth.

community Diffuse hair loss and scalp biopsies

in Research 692 upvotes 4 months ago

Scalp biopsies are crucial for diagnosing hair loss conditions like Diffuse Unpatterned Alopecia (DUPA) and retrograde hair loss, as treatments like finasteride and dutasteride may not be effective if other conditions are present. Combining PPAR-GAMMA agonists with retinoids could improve treatments for conditions like Lichen Planopilaris.

Related Research

1 / 1 results

research Local and Systemic Reduction by Topical Finasteride or Flutamide of Hamster Flank Organ Size and Enzyme Activity

37 citations, November 1995 in “Journal of Investigative Dermatology”

Topical finasteride and flutamide reduce gland size and enzyme activity, with flutamide being more potent, potentially treating acne, seborrhea, hirsutism, and androgenic alopecia.