Generation of Tβ4 Knock-In Cashmere Goat Using CRISPR/Cas9

January 2019 in “ International journal of biological sciences ”

Xiaocong Li, Hao Fei, Xiao Hu, Hui Wang, Bai Dai, Xiao Wang, Hao Liang, Ming Cang, Dongjun Liu

Tβ4 thymosin beta-4 CRISPR/Cas9 RNA-seq vasoconstriction angiogenesis vascular permeability secondary hair follicles CRISPR RNA sequencing

TLDR Researchers used CRISPR/Cas9 to create a goat with a gene that increased cashmere production by 74.5% without affecting quality.

In 2019, researchers successfully created a Tβ4 knock-in cashmere goat by inserting the Tβ4 gene into the goat's CCR5 locus using CRISPR/Cas9 technology, without the need for screening or fluorescent markers. The genetically modified goat showed a 74.5% increase in cashmere yield while maintaining the fiber's fineness and quality. RNA-seq analysis suggested that Tβ4 may enhance hair growth by influencing vasoconstriction, angiogenesis, and vascular permeability around secondary hair follicles. This advancement has the potential to significantly improve cashmere goat breeding and increase economic efficiency.

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Research cited in this study

2 / 2 results

research Tβ4-Overexpression Based on the PiggyBac Transposon System in Cashmere Goats Alters Hair Fiber Characteristics

18 citations, November 2016 in “Transgenic research”

Overexpressing Tβ4 in cashmere goats improves hair fiber traits and increases cashmere yield.

research Thymosin Beta 4 Increases Hair Growth by Activation of Hair Follicle Stem Cells

98 citations, December 2003 in “The FASEB Journal”

Thymosin β4 promotes hair growth by activating stem cells in hair follicles.

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