Standardization of Hibiscus Rosa-Sinensis Flower by HPTLC, HPLC, and AAS

The study aimed to standardize the extract of Hibiscus rosa-sinensis flowers using qualitative phytochemical screening and quantitative HPTLC analysis of quercetin, an active biomarker. HPTLC analysis found 0.33% quercetin, while HPLC found 0.37% quercetin. Heavy metals and trace elements were measured using atomic absorption spectroscopy (AAS), showing that levels of cadmium, lead, arsenic, copper, chromium, and mercury were within acceptable limits. This standardization method ensures better efficacy and safety for raw materials and finished products.