A Method for Simultaneous Analysis of Androgens, Dutasteride, and Finasteride in Human Serum by Liquid Chromatography Tandem Mass Spectrometry

    March 2012 in “ Society for Endocrinology BES 2012 ”
    Rita Upreti, Natalie Homer, Gregorio Naredo, Brian R. Walker, Ruth Andrew
    TLDR A new method was developed to analyze certain hormones and drugs in human blood efficiently.
    The study developed a novel assay using liquid chromatography tandem mass spectrometry to simultaneously analyze testosterone, epitestosterone, DHT, androstenedione, dutasteride, and finasteride in human serum. The method utilized positive atmospheric pressure chemical ionization to avoid matrix-induced ion suppression and optimized conditions for detecting DHT, the least abundant analyte. Chromatographic separation was achieved using a Kinetex C18 column, and several extraction methods were tested, with supported liquid extraction proving most effective for endogenous DHT. This method allowed for the detection of androgens in just 200 µl of male serum and aimed to serve as a research tool for assessing compliance with and biochemical effects of 5α-reductase inhibitors. There were no conflicts of interest or specific funding for this research.
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