Screening and Confirmation Methods of the Major Urinary Metabolite of Finasteride–Carboxy-Finasteride by Liquid Chromatography–Mass Spectrometry and Liquid Chromatography–Tandem Mass Spectrometry
March 2012
in “
Acta Pharmaceutica Sinica B
”
TLDR New methods can detect finasteride's major urinary metabolite in urine for up to 49 hours.
The study developed and validated a sensitive method for detecting carboxy-finasteride, the major urinary metabolite of finasteride, using LC–MS and LC–MS/MS, achieving a limit of detection of 2 ng/mL. This method addressed challenges in doping control, as finasteride is used as a masking agent, and demonstrated good precision and accuracy. It was successfully applied to urine samples from a male volunteer, showing carboxy-finasteride elimination peaked at 10 hours post-administration. The method met WADA requirements and was suitable for routine analysis to detect finasteride misuse in sports.
