A Rapid Screening LC-MS/MS Method Based on Conventional HPLC Pumps for the Analysis of Low Molecular Weight Xenobiotics: Application to Doping Control Analysis
July 2010
in “
Drug Testing and Analysis
”
TLDR A quick method was developed to detect banned substances in urine for doping control.
The study developed a rapid screening method using LC-MS/MS for detecting low molecular weight xenobiotics in urine, specifically for doping control. The method allowed for the analysis of multiple substance classes in a single 11-minute run using conventional HPLC instruments. It was tested on 20 urine samples spiked with 45 prohibited compounds, including diuretics, glucocorticoids, stimulants, and anti-oestrogens. The method demonstrated specificity and sensitivity, with detection limits between 5 ng/mL and 350 ng/mL, below the WADA's required levels. All analytes were distinguishable, including those with similar retention times and fragmentation patterns.
