A Fast Liquid Chromatographic/Mass Spectrometric Screening Method for the Simultaneous Detection of Synthetic Glucocorticoids, Some Stimulants, Anti-Oestrogen Drugs and Synthetic Anabolic Steroids
October 2006
in “
Rapid Communications in Mass Spectrometry
”
TLDR A quick method effectively detects banned substances in urine.
The document described a fast liquid chromatographic/mass spectrometric (LC/MS/MS) screening method developed for detecting synthetic glucocorticoids, stimulants, anti-oestrogen drugs, and synthetic anabolic steroids in urine. This method allowed for the simultaneous extraction and detection of these substances and their metabolites through a single liquid/liquid extraction step at alkaline pH, following enzymatic hydrolysis. The process was completed in 7 minutes using electrospray ionization and multiple reaction monitoring. The method demonstrated good reproducibility with retention times and relative abundances, and it met the World Anti-Doping Agency's performance limits with detection ranges of 1–30 ng/mL for anti-oestrogens, glucocorticoids, and steroids, and 100–200 ng/mL for stimulants.
