Rps14 Upregulation Promotes Inner Ear Progenitor Proliferation and Hair Cell Regeneration in the Neonatal Mouse Cochlea
September 2022
in “
Research Square (Research Square)
”
RPS14 40S ribosomal subunit hair cell regeneration neonatal mouse cochlea AAV-ie system Lgr5+ supporting cells Wnt signaling pathway organ of Corti lineage tracing RNA sequencing cell cycle-related factors Notch signaling TGF-β signaling ribosomal subunit mouse cochlea AAV-ie supporting cells Wnt pathway Corti cell cycle factors Notch TGF-beta
TLDR Increasing Rps14 helps grow more inner ear cells and repair hearing cells in baby mice.
The study investigates the role of RPS14, a component of the 40S ribosomal subunit, in hair cell regeneration in the neonatal mouse cochlea. Researchers used an AAV-ie system to upregulate Rps14 expression in Lgr5+ supporting cells, which are progenitors capable of regenerating hair cells. The upregulation of Rps14 was found to promote the proliferation of these supporting cells and their differentiation into hair cells by activating the Wnt signaling pathway. Additionally, Rps14 overexpression in vivo led to the regeneration of hair cells in the organ of Corti, with lineage tracing confirming that the new hair cells originated from Lgr5+ progenitors. RNA sequencing analysis indicated that Rps14 regulates cell cycle-related factors and Notch/TGF-β signaling, which are crucial during the proliferation of supporting cells and their reprogramming into hair cells. The study concludes that Rps14 has a potential role in stimulating hair cell regeneration in mammals.
