Rat Hair-Follicle-Associated Pluripotent Stem Cells Can Differentiate Into Atrial or Ventricular Cardiomyocytes in Culture Controlled by Specific Supplementation

    January 2024 in “ PloS one ”
    Nanako Takaoka, Michiko Yamane, Ayami Hasegawa, Koya Obara, Kyoumi Shirai, Ryoichi Aki, Hiroyasu Hatakeyama, Yuko Hamada, Nobuko Arakawa, Manabu Tanaka, Robert M. Hoffman, Yasuyuki Amoh
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    TLDR Rat hair-follicle stem cells can become heart cells with specific supplements.
    In this study, rat hair-follicle-associated pluripotent (HAP) stem cells were successfully differentiated into beating atrial and ventricular cardiomyocytes using specific culture supplements. Atrial cardiomyocytes were induced with isoproterenol, activin A, BMP4, bFGF, and CSA, while ventricular cardiomyocytes were induced with activin A, BMP4, bFGF, IWP4, and VEGF. The differentiated cells were confirmed by specific staining and qPCR, showing significant expression of MLC2a in atrial and MLC2v in ventricular cardiomyocytes. Both cell types exhibited characteristic Ca2+ waveforms and formed functional myocardial fibers, demonstrating the potential of HAP stem cells to generate specific cardiomyocyte subtypes.
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