Quantitative Determination of Alpha-Tocopherol and Alpha-Tocopheryl Acetate in Pharmaceutical and Supplementary Soft Capsules by High Performance Liquid Chromatography
August 2025
in “
İstanbul Gelişim Üniversitesi Sağlık Bilimleri Dergisi
”
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Studysummary In this study, the researchers developed a chromatographic method to determine α-tocopherol and α-tocopheryl acetate in pharmaceutical capsules with high accuracy and repeatability, establishing it as a reliable alternative for analyzing these compounds.
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This study successfully determined the amounts of α-tocopherol and α-tocopheryl acetate in pharmaceutical and supplementary soft capsules using a high-performance liquid chromatography method. The method demonstrated high accuracy and repeatability, with recovery values above 95% and repeatability (%RSD) below 3%. The linear regression coefficient (R²) was 0.999, indicating excellent linearity. The limits of detection and quantification for α-tocopherol were 0.131 µg/mL and 0.396 µg/mL, respectively, and for α-tocopheryl acetate, they were 0.260 µg/mL and 0.787 µg/mL. This validated method is a reliable alternative for analyzing these compounds in pharmaceutical products.
