The Prospect and Application Research Progress of Precision Gene Editing Technology Based on CRISPR/Cas9 in Livestock Genetics and Breeding
December 2023
TLDR CRISPR/Cas9 gene-editing shows promise for livestock breeding but faces challenges like low efficiency and off-target effects.
The document reviews the progress and application prospects of CRISPR/Cas9 gene-editing technology in livestock genetics and breeding, particularly focusing on sheep and goats. It highlights the significant advancements made in producing gene-edited livestock, such as pigs, cattle, and sheep, through precise modifications at the genome level. Despite its widespread use, challenges remain, including low insertion efficiency of foreign genes and off-target effects, which limit its application in large livestock like cashmere goats. The review aims to provide a theoretical reference for further research in livestock gene function analysis, genetic improvement, and breeding with local economic characteristics.
