Primary Cell Culture and Morphological Characterization of Canine Dermal Papilla Cells and Dermal Fibroblasts

    February 2002 in “ Veterinary Dermatology ”
    Christine B. Bratka‐Robia, Gerda Mitteregger, Amanda Aichinger, Monika Egerbacher, M. Helmreich, E. Bamberg
    TLDR Canine dermal papilla cells and fibroblasts have distinct growth patterns and protein expressions.
    The study focused on the primary cell culture and morphological characterization of canine dermal papilla cells (DPC) and dermal fibroblasts (DFB) from female dogs. DPCs were isolated from primary hair follicles and cultured in Amniomax complete C100 medium, reaching confluence in 3 weeks and subcultivated every 7 days. DFBs were cultured from interfollicular dermis in serum-free medium, reaching confluence in 10 days and subcultivated every 5 days. Immunohistochemistry revealed that DPCs exhibited an aggregative growth pattern with pseudopapillae formation and strong staining for collagen IV and laminin up to the sixth passage, while DFBs grew in branching, parallel lines with weak staining for these proteins.
    Discuss this study in the Community →

    Research cited in this study

    13 / 13 results

    Related Community Posts Join

    6 / 1000+ results

      community Looks like a real cure to the root of baldness (DHT-induced senescence) was proven earlier this year

      in Research/Science  733 upvotes 3 years ago
      A study that outlines the full model for androgenic alopecia (AGA) which links DHT to cellular senescence in dermal papilla cells, and suggests black chokeberry as a source of cyanidin 3-O-arabinoside polyphenol with potential anti-oxidant properties that could reverse this process. The post encourages reaching out to experts in anti-aging and longevity to research treatments involving the polyphenol.

      community Comprehensive Microneedling Guide

      in Microneedling  295 upvotes 3 years ago
      Microneedling for hair restoration involves daily use of a 0.3 mm device and weekly use of a 0.5 mm device. Combining microneedling with Minoxidil can enhance hair growth.

      community Verteporfin and Microneedling

      in Treatments 1 year ago
      Clinical studies by Dr. Barghouthi and Dr. Bloxham indicate that Verteporfin, when used with FUE and FUT hair transplantation methods, shows promise in hair follicle regeneration and minimal scarring due to its ability to inhibit Yes-associated protein (YAP). Microneedling at depths of 3-3.5mm, combined with Verteporfin, could potentially reactivate dormant follicles, although the optimal dosage and application method are still under investigation. Concerns remain about the DHT sensitivity of regenerated follicles, highlighting the need for further research to optimize trauma levels and Verteporfin concentrations to achieve effective and scar-free hair regeneration.

      community This is the best time in human history to be fighting hair loss

      in Treatment  194 upvotes 3 years ago
      This conversation discusses the advancements in treatments for hair loss, including finasteride, dutasteride, minoxidil and new promising treatments such as pyrilutamide and verteporfin. Other treatments discussed were taking minoxidil orally, tretinoin to turn non-responders into responders and microneedling to further boost growth.

    Related Research

    1 / 1 results