Pectin Nanoparticle-Loaded Soft Coral Nephthea sp. Extract as In Situ Gel Enhances Chronic Wound Healing: In Vitro, In Vivo, and In Silico Studies
July 2023
in “
Pharmaceuticals
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TLDR The gel with coral extract and pectin nanoparticles helps heal chronic wounds.
This study explores the wound healing potential of a Nephthea sp. extract loaded with pectin nanoparticles (LPNs) in an in situ gel formulation, using a diabetic excision wound model. The research identified 27 secondary metabolites and reported the isolation of specific acids and DEHP for the first time. The LPN in situ gel effectively regulated proinflammatory cytokines (NF-κB, TNF-α, IL-6, and IL-1β) on days 7 and 15. In vitro tests showed the extract's inhibitory effects on GSK-3 and MMP-1, with IC50 values of 0.178 ± 0.009 and 0.258 ± 0.011 µg/mL, respectively. Molecular docking revealed strong binding affinities for chabrolosteroid E and isogosterone B with GSK-3 and MMP-1. Pharmacokinetics studies predicted the wound healing activity's mode of action, highlighting the potential of this marine-derived nanoparticle gel in chronic wound treatment.
