Development of a New Gas Chromatography–Mass Spectrometry Methodology for the Evaluation of 5α-Reductase Activity
January 2013
in “
Talanta
”
TLDR A new method was developed to accurately measure enzyme activity related to prostate health.
The study developed and validated a new gas chromatography–mass spectrometry (GC–MS) methodology using isotopic dilution dispersive liquid–liquid microextraction (DLLME) to evaluate 5α-reductase (5α-R) activity by measuring the conversion of testosterone (T) into dihydrotestosterone (DHT) in human prostatic microsomes. This method was found to be highly sensitive, accurate, and precise, with good linearity, allowing for the simultaneous separation, identification, and quantification of T and DHT. The DLLME technique provided a simple and fast sample preparation process, making it a valuable tool for evaluating new compounds as potential 5α-reductase inhibitors, which could contribute to the treatment of benign prostate hyperplasia (BPH) and prostate cancer chemoprevention.
