Investigation of the Mitigation of DMSO-Induced Cytotoxicity by Hyaluronic Acid Following Cryopreservation of Human Nucleus Pulposus Cells

The study explored the use of hyaluronic acid (HA) to reduce dimethyl sulfoxide (DMSO)-induced cytotoxicity in cryopreserved human nucleus pulposus cells (NPCs). Involving 7 samples per group, the research compared NPCs mixed with either EDTA-PBS or HA. Results indicated that HA significantly improved cell proliferation and increased the number of Tie2-positive cells, which are important for intervertebral disc repair. HA also reduced oxidative stress caused by DMSO. These findings suggest that HA could enhance the development of NPC-based transplantation products by maintaining cell viability and functionality, although further in vivo research is needed.