Short-Term Cryoprotectant-Free Cryopreservation at −20°C Does Not Affect the Viability and Regenerative Capacity of Nanofat

    E. Limido, Andrea Weinzierl, Emmanuel Ampofo, Yves Harder, Michael D. Menger, Matthias W. Laschke
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    TLDR Storing nanofat at -20°C for 7 days does not harm its ability to regenerate.
    Cryopreservation of nanofat at -20°C for 7 days does not affect its viability or regenerative capacity, as evidenced by comparable apoptotic rates, blood and lymph vessel densities, and wound healing outcomes to freshly generated nanofat. This finding suggests that nanofat can be effectively stored for repeated use without losing its regenerative properties, potentially expanding its applications.
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