New Hyaluronan-Based Biomatrix for 3D Follicle Culture Yields Functionally Competent Oocytes

The study investigated a novel tyramine-linked hyaluronan gel for 3-D in vitro culture of mouse ovarian follicles, aiming to produce developmentally competent metaphase II oocytes. Out of 402 preantral follicles cultured, 73% matured to metaphase II, with 83% fertilizing and a 46% blastulation rate. The hyaluronan gel proved effective for follicle culture, supporting the ovulation of functionally competent oocytes. The study also explored modifications with different extracellular matrices, which influenced antrum formation but not maturation rates significantly. The research suggested potential future applications for human follicles, offering a promising non-surgical approach for deriving mature oocytes from cryopreserved ovarian tissue, potentially benefiting cancer patients by reducing the risk of reintroducing malignant cells.