High-Throughput Screening of 3D Co-Culture Hair Follicle Mimetic Tissue with Enhanced ECM for the Screening of Hair Growth Promoting Compounds

January 2024 in “ Biomaterials Research ”

Huyen T. M. Pham, Hyo-Sop Kim, Duc Long Nguyen, Hyun Woo Joo, Min Kyu Kim, Young Kwan Sung, Duc Vu, Heung Sik Hahm, Se Joo Kim, Jae‐Ho Kim, Hyun‐Ji Park

3D co-culture hair follicle extracellular matrix ECM dermal papilla cells keratinocytes spheroids epithelial-mesenchymal interactions

TLDR The new 3D system helps test hair growth treatments effectively.

The study introduces a novel 3D co-culture system that mimics the in vivo extracellular matrix (ECM) dynamics crucial for hair follicle development and regeneration. This system uses primary dermal papilla cells from human patients co-cultured with neonatal keratinocytes, forming uniform spheroids that closely resemble native ECM structures. The model supports high-throughput screening, allowing for the evaluation of approximately 60 spheroids per well, and demonstrates consistent cellular organization and enhanced ECM-related gene expression. This platform provides a robust and reproducible method for assessing potential hair loss treatments, advancing the development of therapeutic candidates through effective epithelial-mesenchymal interactions.

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