Lentiviral Arrays for Live-Cell Dynamic Monitoring of Gene and Pathway Activity During Stem Cell Differentiation

June 2014 in “ Molecular therapy ”

Roshan Padmashali, Panagiotis Mistriotis, Mao-Shih Liang, Stelios T. Andreadis

mesenchymal stem cells hair follicle MSCs FGF-R PI3K glucocorticoid-R TGF-β ERK p38 MSCs hair follicle stem cells fibroblast growth factor receptor phosphoinositide 3-kinase glucocorticoid receptor transforming growth factor beta extracellular signal-regulated kinase p38 MAP kinase

TLDR The lentiviral array can monitor and predict gene activity during stem cell differentiation.

The 2014 study developed a lentiviral array (LVA) system to monitor gene and pathway activity in real-time during the differentiation of mesenchymal stem cells (MSCs) into adipogenic, chondrogenic, and osteogenic lineages. The LVA system identified key pathways such as FGF-R, PI3K, and glucocorticoid-R, which were essential for all lineages, and highlighted the differential roles of pathways like TGF-β, ERK, and p38. The study involved human bone marrow MSCs and hair follicle MSCs, using fluorescence microscopy, staining methods, and western blot analysis to validate findings. This approach provided valuable insights into the temporal regulation of gene expression and pathway activation, aiding in the discovery of novel differentiation inducers and genes involved in lineage specification.

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Research cited in this study

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Clonal Multipotency and Effect of Long-Term In Vitro Expansion on Differentiation Potential of Human Hair Follicle Derived Mesenchymal Stem Cells

research Clonal Multipotency and Effect of Long-Term In Vitro Expansion on Differentiation Potential of Human Hair Follicle Derived Mesenchymal Stem Cells

73 citations, August 2011 in “Stem Cell Research”

Human hair follicle stem cells can turn into multiple cell types but lose some of this ability after being grown in the lab for a long time.

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