Lentiviral Arrays for Live-Cell Dynamic Monitoring of Gene and Pathway Activity During Stem Cell Differentiation

June 2014 in “ Molecular therapy ”

Roshan Padmashali, Panagiotis Mistriotis, Mao-Shih Liang, Stelios T. Andreadis

mesenchymal stem cells hair follicle MSCs FGF-R PI3K glucocorticoid-R TGF-β ERK p38 MSCs hair follicle stem cells fibroblast growth factor receptor phosphoinositide 3-kinase glucocorticoid receptor transforming growth factor beta extracellular signal-regulated kinase p38 MAP kinase

TLDR The lentiviral array can monitor and predict gene activity during stem cell differentiation.

The 2014 study developed a lentiviral array (LVA) system to monitor gene and pathway activity in real-time during the differentiation of mesenchymal stem cells (MSCs) into adipogenic, chondrogenic, and osteogenic lineages. The LVA system identified key pathways such as FGF-R, PI3K, and glucocorticoid-R, which were essential for all lineages, and highlighted the differential roles of pathways like TGF-β, ERK, and p38. The study involved human bone marrow MSCs and hair follicle MSCs, using fluorescence microscopy, staining methods, and western blot analysis to validate findings. This approach provided valuable insights into the temporal regulation of gene expression and pathway activation, aiding in the discovery of novel differentiation inducers and genes involved in lineage specification.

View this study on cell.com →

Discuss this study in the Community →

Research cited in this study

1 / 1 results

Clonal Multipotency and Effect of Long-Term In Vitro Expansion on Differentiation Potential of Human Hair Follicle Derived Mesenchymal Stem Cells

research Clonal Multipotency and Effect of Long-Term In Vitro Expansion on Differentiation Potential of Human Hair Follicle Derived Mesenchymal Stem Cells

73 citations, August 2011 in “Stem Cell Research”

Human hair follicle stem cells can turn into multiple cell types but lose some of this ability after being grown in the lab for a long time.

Related Community Posts Join

6 / 13 results

community Microneedling and aloe vera to recruit stem cells.

in Chat 11 upvotes 10 months ago

Microneedling combined with aloe vera may stimulate dormant stem cells in hair loss areas. Minoxidil is also suggested for better results.

community The Real Cause Of Androgenetic Alopecia

in Research/Science 667 upvotes 2 months ago

Androgenetic alopecia is caused by DHT affecting hair growth. Finasteride and minoxidil are used to manage hair loss by blocking DHT and promoting hair growth.

community New and Interesting HairLoss Studies/Papers/Reviews

in Treatment 42 upvotes 4 years ago

Hair loss treatments discussed include Dutasteride with Ketoconazole, tissue engineering strategies, and androgenetic alopecia therapies. Massage doubles follicular retention, improving treatment effectiveness.

community Anyone Try Microneedling with Exosomes/AAPE?

in Microneedling 2 upvotes 2 years ago

User asks where to purchase exosomes/AAPE for microneedling hair loss treatment. Links to research on hair regeneration therapy, stem cell therapy, exosome therapy, and dermal exosomes provided.

community TWIST-1 and the cure: Is it really that easy?

in Research/Science 283 upvotes 2 years ago

TWIST-1 gene's role in hair loss and potential as a treatment target. Inhibiting TWIST-1 may prolong hair growth and reduce hair follicle sensitivity to DHT.

community Diffuse hair loss and scalp biopsies

in Research 692 upvotes 4 months ago

Scalp biopsies are crucial for diagnosing hair loss conditions like Diffuse Unpatterned Alopecia (DUPA) and retrograde hair loss, as treatments like finasteride and dutasteride may not be effective if other conditions are present. Combining PPAR-GAMMA agonists with retinoids could improve treatments for conditions like Lichen Planopilaris.