A Liquid Chromatography/Tandem Mass Spectrometry Method for Determination of Aristolochic Acid-I in Rat Plasma
July 2009
in “
Biomedical Chromatography
”
New to Finasteride? There is a guide in the encyclopedia. Read the guide → Studysummary This study developed and validated a sensitive liquid chromatography–mass spectrometry method to measure aristolochic acid‐I in rat plasma, successfully applying it to pharmacokinetic research.
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The study developed and validated a sensitive and specific liquid chromatography–tandem mass spectrometry method for determining aristolochic acid‐I (AA‐I) in rat plasma, using finasteride as an internal standard. The method involved isocratic elution on a Zorbax Eclipse XDB‐C 18 column and mass spectrometry in positive multiple reaction monitoring mode, achieving good linearity over 0.4–600 ng/mL. The method demonstrated intra- and inter-day precisions with relative standard deviations of less than 13.5% and accuracy between 94.2% and 97.5%. It was successfully applied to study the pharmacokinetics of AA‐I in rats.
