A Method to Monitor Dutasteride in Rat Plasma Using Liquid-Liquid Extraction and Multiple Reaction Monitoring: Comparisons and Validation

The study compared three extraction methods for dutasteride from rat plasma and found that liquid-liquid extraction using methyl tert-butyl ether and methylene chloride was the most effective. The method, combined with liquid chromatography and multiple reaction monitoring, was validated for specificity, linearity (r² = 0.9993, 5-400 ng/mL), sensitivity (detection limit: 4.03 ng/mL; quantitation limit: 12.10 ng/mL), accuracy (intra-day: 89.4-105.9%; inter-day: 84.9-100.9%), precision (intra-day: 0.8-6.9%; inter-day: 2.9-15.9%), and recovery (84.7-107.8%). This validated method was successfully applied to a pharmacokinetic study of dutasteride, suggesting its utility for evaluating new dutasteride formulations.