A Method to Monitor Dutasteride in Rat Plasma Using Liquid-Liquid Extraction and Multiple Reaction Monitoring: Comparisons and Validation
September 2014
in “
Mass spectrometry letters
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TLDR The best method to monitor dutasteride in rat plasma is liquid-liquid extraction with methyl tert-butyl ether and methylene chloride.
The study compared three different methods for extracting dutasteride from rat plasma and found that liquid-liquid extraction using methyl tert-butyl ether and methylene chloride was the most effective. The method, which included liquid chromatography and multiple reaction monitoring, was validated for specificity, linearity (r² = 0.9993, 5-400 ng/mL), sensitivity, accuracy, precision, and recovery. The validated method was successfully applied to a pharmacokinetic study of dutasteride, suggesting its usefulness for evaluating new dutasteride formulations.
