Generating LacZ-Reporter Transgenic Mice to Identify Alpha1 (XIX) Collagen (Col19a1) Expression in Dermal Papilla Cells
March 2016
in “
Benha Veterinary Medical Journal
”
TLDR Type XIX Collagen is present in specific skin and hair cells during development.
The study focused on understanding the role of type XIX Collagen (Col19a1) in hair follicle morphogenesis by generating Col19a1LacZ reporter mice using the CRISPR/Cas9 system. The expression of Col19a1 was observed in dermal papilla cells of hair follicles, whisker ring sinuses, skeletal muscles, and the basal keratinocyte layer of the skin in mouse embryos. Col19a1 expression began at the E14.5 stage in dermal condensates and continued in mature hair follicle dermal papilla cells, but was absent in the placode stage. The research aimed to explore the functional role of Col19a1 in hair follicle development, with the expectation that future studies would provide further insights into its role in hair follicle stem cell regulation.
