Rapid Generation of Homogenous Tumor Spheroid Microtissues in a Scaffold-Free Platform for High-Throughput Screening of a Novel Combination Nanomedicine

This study developed a new mask-free method using digital micromirror and microfluidic systems to create multicellular heterospheroids for drug screening, finding that heterospheroids exhibit higher drug resistance and combinatorial drugs are more effective than single drugs in cancer therapeutic applications.

This study designed a micromold template for creating controlled-sized, multicellular tumor spheroids, finding that HEK-293 cells formed consistent spheroids with high circularity and viability after 7 days, influenced by cell seeding density and treatment conditions.

This study demonstrates that a 3D-printed agarose microwell platform supports the growth of size-uniform, viable NSCLC cancer spheroids, and can effectively evaluate drug responses using limited tumor material from biopsies, particularly with EGFR tyrosine kinase inhibitors gefitinib and osimertinib.

This review highlights recent advancements in microfluidics for 3D tissue model generation and discusses its applications in tissue engineering and high-throughput drug screening without reporting new experimental results.

In this study, the 40-MBR system was shown to enhance drug-screening efficiency by providing reliable, real-time monitoring of cell conditions in 3-D cultures compared to traditional methods.