Development of 3D Microbioreactor Systems for Cell-Based High Throughput Screening

This mini-review reports that advances in microbioreactor technology, particularly with microfluidics, significantly enhance eukaryotic cell-based biomedicine by improving productivity, scalability, and reproducibility in applications like CAR T-cell manufacturing and organoid growth, though challenges in scalability and material selection remain.

This review highlights recent advancements in microfluidics for 3D tissue model generation and discusses its applications in tissue engineering and high-throughput drug screening without reporting new experimental results.

This review highlights the current progress and application pros and cons of various cell-based screening platforms in drug discovery, discussing challenges and potential improvements for these innovative methods.

This study designed a micromold template for creating controlled-sized, multicellular tumor spheroids, finding that HEK-293 cells formed consistent spheroids with high circularity and viability after 7 days, influenced by cell seeding density and treatment conditions.

This study developed a new mask-free method using digital micromirror and microfluidic systems to create multicellular heterospheroids for drug screening, finding that heterospheroids exhibit higher drug resistance and combinatorial drugs are more effective than single drugs in cancer therapeutic applications.