Isolating Hair Follicle Stem Cells and Epidermal Keratinocytes from Dorsal Mouse Skin

April 2016 in “ Journal of Visualized Experiments ”

Despina Soteriou, Lana Kostic, Egor Sedov, Yahav Yosefzon, Hermann Steller, Yaron Fuchs

hair follicle stem cells epidermal keratinocytes fluorescence activated cell-sorting CD34 keratin 15 bulge region in vivo grafting in vitro studies multipotency quiescence activation FACS K15

The document described a method for isolating hair follicle stem cells (HFSCs) and epidermal keratinocytes from mouse hair follicles using fluorescence activated cell-sorting (FACS) technology. These stem cells, located in the bulge region of the hair follicle, were identified by specific markers such as CD34 and keratin 15 (K15). The isolated HFSCs were valuable for in vivo grafting models and in vitro studies to understand the mechanisms of multipotency, quiescence, and activation in adult stem cells.

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