Inhibition of the Pro-Oxidant Effects of Benoxaprofen on Polymorphonuclear Leucocytes by Retinol Acetate In Vitro

The document discusses the pro-oxidant effects of benoxaprofen, a non-steroidal anti-inflammatory drug, on human polymorphonuclear leucocytes (PMNL) and the potential for retinol acetate to inhibit these effects. The study found that benoxaprofen stimulates PMNL oxidative metabolism and lysosomal enzyme release in vitro, which can be decreased by retinol acetate but not by tocopherol acetate. This suggests that benoxaprofen may sensitize the skin to sunlight and contribute to side effects like photosensitivity and onycholysis. Retinol acetate, a lipophilic antioxidant, could be clinically valuable in controlling or preventing these effects. The study used PMNL obtained from heparinized venous blood, with benoxaprofen at a concentration of 10^-4M and retinol acetate at concentrations of 10^-4M and 10^-5M. Chemiluminescence and degranulation were measured, showing that retinol acetate decreased the pro-oxidant activities induced by benoxaprofen. The results are based on the mean value and standard error of three different experiments.