Advantages of Incomplete Digestion in Human Hair Shaft Proteomics: A Focus on Cuticular Keratins

    June 2025 in “ Rapid Communications in Mass Spectrometry ”
    Phonchawit Kiatthitinan, D. Yee, Huu M. Tran, Bingyun Sun
    Studysummary In this study, researchers developed a simplified and reliable method to prepare human hair shaft samples, achieving over 75% protein extraction efficiency and improved keratin sequence coverage, with the approach showing high reproducibility across different labs and operators.
    Automatically generated from the study's abstract, not written by a person, and not a review of the full paper. Not medical advice or a treatment recommendation. Read the original study, and consult a qualified healthcare professional before changing treatment. Full disclaimer
    Read the full study on onlinelibrary.wiley.com →
    Discuss this study in the Community →

    Research cited in this study 13

    1. A Methodological Exploration of Distinguishing Hair Quality Based on Hair Proteomics Proteome science · 2024
    2. Optimal Processing for Proteomic Genotyping of Single Human Hairs Forensic Science International Genetics · 2020
    3. Age-Related Changes in Hair Shaft Protein Profiling and Genetically Variant Peptides Forensic Science International Genetics · 2020
    4. Proteomic Characterization of Damaged Single Hairs Recovered After an Explosion for Protein-Based Human Identification Journal of proteome research · 2020
    5. Nanoindentation of Hair Cortex and Medulla Regions Fibers And Polymers/Fibers and polymers · 2019
    6. Variation in Human Hair Ultrastructure Among Three Biogeographic Populations Journal of structural biology · 2018
    7. Protein Extraction from Human Anagen Head Hairs 1 Millimeter or Less in Total Length Biotechniques/BioTechniques · 2018
    8. The Structure of People's Hair PeerJ · 2014
    9. The Keratins of the Human Beard Hair Medulla: The Riddle in the Middle ˜The œjournal of investigative dermatology/Journal of investigative dermatology · 2009
    10. The Hair Follicle as a Dynamic Mini-Organ Current Biology · 2009
    11. Hair Cuticle Differences Between Asian And Caucasian Females International Journal of Dermatology · 2006
    12. The Catalog of Human Hair Keratins Journal of biological chemistry/˜The œJournal of biological chemistry · 1999
    13. Human Hair Keratins Journal of Investigative Dermatology · 1993

    Related research 3

    1. Keratins and Lipids in Ethnic Hair International Journal of Cosmetic Science · 2013
    2. Unzipping the Cuticle of the Human Hair Shaft to Obtain Micron/Nano Keratin Filaments Biopolymers · 2006
    3. Human Hair Keratin-Associated Proteins ˜The œjournal of investigative dermatology. Symposium proceedings/˜The œJournal of investigative dermatology symposium proceedings · 2005