HPTLC Assay of Nicotine and Cotinine in Biological Samples

    October 2018 in “ Journal of Mind and Medical Sciences ”
    Ana Vlasceanu, Daniela Luiza Baconi, Anne Marie Ciobanu, Vlad Denis Constantin, Liana Pleș, Cristian Bălălău, Miriana Stan
    TLDR A new method effectively measures nicotine and cotinine in blood and urine for forensic analysis.
    The study developed a high-performance thin-layer chromatography (HPTLC) method to identify and semi-quantitatively evaluate nicotine and its metabolite cotinine in human plasma and urine. The method achieved the best separation using a methanol: chloroform: ammonia solvent system and found liquid-liquid extraction with dichloromethane at alkaline pH to be more effective than solid-phase extraction. Calibration curves for nicotine and cotinine were linear in the range of 200-1000 ng/spot, with correlation coefficients over 0.99. This method was applicable for detecting nicotine and cotinine at toxic levels, potentially useful in forensic toxicology for cases of nicotine intoxication.
    Discuss this study in the Community →

    Research cited in this study

    1 / 1 results

    Related Community Posts Join

    6 / 112 results

      community High Nicotine use and Hair Loss

      in Chat  91 upvotes 1 year ago
      Heavy nicotine use can lead to hair thinning and loss, but quitting can reverse these effects. The user noticed significant hair regrowth after quitting vaping.

      community Just quit nicotine and caffeine

      in Treatment  20 upvotes 3 years ago
      The user has been using finasteride and oral minoxidil for hair loss but hasn't seen improvement and is quitting nicotine and caffeine to see if it helps. Some believe quitting nicotine improved their hair health due to better blood flow, while others are skeptical about the impact of blood flow on hair loss.

      community How bad is nicotine when it comes to hairloss

       7 upvotes 7 years ago
      The conversation discusses the impact of nicotine and caffeine on hair loss. The user has been using finasteride, minoxidil, and nizoral for hair loss treatment and is questioning whether to stop using nicotine products.

    Similar Research

    5 / 323 results