Ficoll Density Gradient Sedimentation Isolation of Pelage Hair Follicle Mesenchymal Stem Cells from Adult Mouse Back Skin: A Novel Method for Hair Follicle Mesenchymal Stem Cells Isolation

The document presents a novel method for isolating hair follicle mesenchymal stem cells (HF-MSC) from adult mice using Ficoll Density Gradient Sedimentation (FDGS). This method is faster and yields a larger number of cells compared to traditional methods. The isolated cells, known as pelage HF-MSC, were found to express similar markers, show similar growth patterns, and possess similar functions as whisker HF-MSC. When injected into the dorsal skin of mice, these cells promoted hair growth. The cells were distributed around the host hair follicle, secreting exosomes that integrated into the host hair follicle and located in the dermal papillae and hair matrix region. Therefore, the FDGS method can isolate a large number of pelage HF-MSC that can promote hair growth by secreting exosomes targeting the dermal papilla and hair matrix region of the host hair follicle.