Extraction and Detection of mRNA from Horsehair

    January 2006 in “ Journal of veterinary medical science ”
    Tetsuo Sato, Go Sato, Youko Shoji, Takuya Itou, Takeo Sakai
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    TLDR RNA can be extracted from horsehair roots for analysis.
    Researchers successfully extracted RNA from horsehair shafts and roots, detecting mRNAs of beta-actin, muscle-type phosphofructokinase, and transforming growth factor-beta1 using reverse transcription polymerase chain reaction. While RNA amounts were low, mRNA transcripts were detectable with more than three hair roots, but detection was challenging in hair shafts. This indicated that residual RNA in horsehair roots could be used for molecular biological analysis.
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