TLDR Negative pressure treatment improved skin thickness, blood vessel growth, and hair growth.
The study investigated the effects of negative pressure treatment using an External Tissue Expansion Device (ETED) on fat grafting recipient sites in a porcine model. The ETED applied -70 mmHg pressure for 1 or 3 hours every other day, over 10 or 20 treatments. Results showed increased epidermis thickness due to cell proliferation, a more than two-fold increase in vessel density, and an unexpected increase in hair follicles. This suggested that the ETED promoted vascularization and hair growth. The porcine model was deemed more representative for studying the effects of negative pressure on skin tissue compared to smaller animal models.
610 citations
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April 2014 in “Nature Reviews Immunology” The document concludes that understanding how the skin's immune system and inflammation work is complex and requires more research to improve treatments for skin diseases.
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April 2005 in “Experimental dermatology” Prostaglandin F2alpha and related compounds can increase hair growth and darken hair in mice.
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November 2002 in “Journal of Investigative Dermatology” 
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April 2017 in “Scientific Reports” Using a perfusion system and 3D spheroid culture improves the growth of corneal cell layers for tissue engineering.
Elastin-like recombinamers show promise for better wound healing and skin regeneration.
306 citations
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April 2019 in “International Journal of Molecular Sciences” The skin has a complex immune system that is essential for protection and healing, requiring more research for better wound treatment.
