Immunolocalization of Cytokeratins in Some Freshly Fixed Bovine Epithelia

The study investigated cytokeratin expression in various freshly fixed bovine epithelia using the ABC immunoperoxidase method with specific monoclonal antibodies. It found that hair follicles and nasolabial glands expressed both small acidic and large basic cytokeratins, whereas epidermal cells, sebaceous glands, and mucosae contained only large basic cytokeratins, and the mammary gland contained only small acidic cytokeratins. These findings were compared with previous reports on cell cultures from human and bovine specimens.