Cutaneous Transcriptome Analysis in NIH Hairless Mice
August 2017
in “
PloS one
”
hair growth cycle H&E staining RNA-Seq differentially expressed genes basal cell carcinoma cell cycle Hippo signaling pathway Hedgehog signaling pathway Wnt signaling pathway cell proliferation hair follicle development q-PCR Pik3r1 Pik3r3 hematoxylin and eosin staining RNA sequencing quantitative PCR
TLDR Key genes linked to hair growth and cancer were identified in hairless mice.
In this study, researchers examined skin hair growth cycle abnormalities in NIH hairless mice at 42 days old using H&E staining and RNA-Seq, identifying 5,068 differentially expressed genes. Pathway analysis revealed up-regulation in basal cell carcinoma, cell cycle, and Hippo, Hedgehog, and Wnt signaling pathways, which are linked to cell proliferation and cancer development, while signal transduction and infection-related pathways were down-regulated. Gene interaction network analysis highlighted genes related to hair follicle development, and q-PCR confirmed the RNA-Seq results. Key genes Pik3r1 and Pik3r3 were identified, providing a basis for further research on hairless mice.
