CCL5 Affects the Hair-Inductive Capacity of Three-Dimensional Cultured Dermal Papilla Cells
May 2017
in “
Journal of Investigative Dermatology
”
C-C motif ligand 5 CCL5 three-dimensional cultured dermal papilla cells 3D-cultured dermal papilla cells dermal papilla cells DP cells alkaline phosphatase ALP hair growth potential OVOL1 hair follicle induction small interfering RNA siRNA epidermal cells 3D-cultured DP cells hair growth hair follicle skin cells
TLDR CCL5 is important for the hair growth potential of human dermal papilla cells.
The document reports on a study that investigated the role of C-C motif ligand 5 (CCL5) in the hair-inductive capacity of three-dimensional (3D)-cultured human dermal papilla (DP) cells. Alkaline phosphatase (ALP) is a signature gene of DP cells, and its activity is associated with the hair growth potential of these cells. The study found that CCL5 was down-regulated when ALP was ablated in DP spheres. Given that CCL5 had been previously linked to hair growth through its upregulation by OVOL1, the researchers explored whether CCL5 influences the hair growth potential of human DP spheres. They discovered that hair follicle induction was significantly impaired when DP spheres transfected with CCL5 small interfering RNA (siRNA) were co-implanted with epidermal cells. This suggests that ALP-regulated expression of CCL5 contributes to the hair growth potential of human DP spheres.
