Effects of Anthraquinone on Inducing Cell Cycle Arrest and Apoptosis in Human Malignant Melanoma Cell Line (A375.S2)
January 2008
in “
中山醫學大學醫學研究所學位論文
”
Danthron Rhubarb malignant melanoma A375.S2 cell cycle arrest G0/G1 phase apoptosis DNA damage mitochondrial membrane potential cytochrome c AIF Endo G Bax Bcl-xL Bcl-2 caspase-9 caspase-3 intracellular calcium reactive oxygen species mitochondrial pathway endoplasmic reticulum pathway death receptor pathway Rhubarb extract
TLDR Danthron from Rhubarb causes melanoma cells to stop growing and die.
The study investigated the effects of Danthron, a compound from Rhubarb, on the human malignant melanoma cell line A375.S2. It was found that Danthron induced cell cycle arrest at the G0/G1 phase and promoted apoptosis through both caspase-dependent and caspase-independent pathways. The mechanisms involved included DNA damage, decreased mitochondrial membrane potential, release of cytochrome c, AIF, and Endo G, and changes in the expression of apoptosis-related proteins such as Bax, Bcl-xL, and Bcl-2. Additionally, Danthron increased intracellular calcium concentration and reactive oxygen species, further contributing to apoptosis via mitochondrial, endoplasmic reticulum, and death receptor pathways.
