Effects of Anthraquinone on Inducing Cell Cycle Arrest and Apoptosis in Human Malignant Melanoma Cell Line (A375.S2)
January 2008
in “
中山醫學大學醫學研究所學位論文
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Danthron Rhubarb malignant melanoma A375.S2 cell cycle arrest G0/G1 phase apoptosis DNA damage mitochondrial membrane potential cytochrome c AIF Endo G Bax Bcl-xL Bcl-2 caspase-9 caspase-3 intracellular calcium reactive oxygen species mitochondrial pathway endoplasmic reticulum pathway death receptor pathway Rhubarb extract
Studysummary This study found that Danthron induced apoptosis in a human malignant melanoma cell line through mitochondrial, endoplasmic reticulum, and death receptor pathways. Our plain-language summary of this paper — not a Tressless recommendation.
The study investigated the effects of Danthron, a compound from Rhubarb, on the human malignant melanoma cell line A375.S2. It was found that Danthron induced cell cycle arrest at the G0/G1 phase and promoted apoptosis through both caspase-dependent and caspase-independent pathways. The mechanisms involved included DNA damage, decreased mitochondrial membrane potential, release of cytochrome c, AIF, and Endo G, and changes in the expression of apoptosis-related proteins such as Bax, Bcl-xL, and Bcl-2. Additionally, Danthron increased intracellular calcium concentration and reactive oxygen species, further contributing to apoptosis via mitochondrial, endoplasmic reticulum, and death receptor pathways.
