Effects of Different Concentrations of Androgens on KAP24.1 Gene Expression in Hetian Sheep and Karakul Sheep

The study successfully cloned the complete CDS region of the KAP24.1 gene from plain-type Hetian sheep, mountain-type Hetian sheep, and Karakul sheep, with a length of 759 bp encoding 252 amino acids. The KAP24.1 gene was found to be an unstable protein. Sequence similarity and phylogenetic tree analysis indicated that these sheep have the closest genetic relationship with goats and the farthest with Canadian deer. Additionally, the eukaryotic recombinant expression vector of the KAP24.1 gene was successfully constructed, providing a basis for further research on the gene's role in determining wool traits.