ABO Blood Grouping of Non-Medullated Scalp Hair Using Immunohistochemical Staining

    January 2010 in “ Nihon Hou Kagaku Gijutsu Gakkaishi ”
    Tomoko Akutsu, Hiroshi Ikegaya, Ken Watanabe, Koichi Sakurada
    Image of study
    TLDR The modified staining method can determine the ABO blood group of hair samples accurately.
    In this study, researchers modified the immunohistochemical staining protocol to determine the ABO blood group of non-medullated scalp hair. They found that using 3,3',5,5'-tetramethylbenzidine as a substrate resulted in positive staining of the hair cortex, but non-specific staining in medullated hair. This issue was mitigated by blocking with 10% normal rabbit serum at 37°C for 1 hour. In a blind trial, the modified method successfully determined the ABO blood group of all hair samples. The study concluded that this protocol is useful for screening hair samples before DNA analysis.
    Discuss this study in the Community →

    Related Community Posts Join

    2 / 2 results

      community Another clueless guy fearmongering and spreading misinformation

      in Chat  251 upvotes 9 months ago
      The conversation discusses hair loss treatments, with some users advocating for finasteride and minoxidil, while others express concerns about potential side effects of finasteride. A social media influencer is criticized for promoting scalp massages and minoxidil over finasteride, which some believe is misinformation.

    Similar Research

    5 / 1000+ results