Production of a 135-Residue Long N-Truncated Human Keratinocyte Growth Factor 1 in Escherichia Coli
May 2023
in “
Microbial Cell Factories
”
TLDR A stable, active version of a growth factor was made in bacteria, showing promise for medical use.
The study successfully produced a 135-residue N-truncated variant of human keratinocyte growth factor 1 (KGF-1135) in Escherichia coli, demonstrating its potential as a stable and biologically active alternative to the full-length KGF-1. The KGF-1135 variant was fused with thioredoxin to enhance production yield and was purified to over 99% purity. It maintained a structure similar to the original KGF-1 and exhibited higher thermal stability. Biological activity was confirmed through MCF-7 cell proliferation assays, showing similar activity to full-length KGF-1 and prolonged activation of signaling pathways. The study suggests KGF-1135 could be used in therapeutic applications such as hair growth, wound healing, and cancer treatment, although production yield remains a challenge.
